Resources

Protocols

Organoid Culture

• Mouse Colon Organoid Culture

• Human Colon Organoid Culture

• Organoid Immunofluorescence

• Organoid Translational Technology Platform

Mass Cytometry (CyTOF)

• Multiplexed Single-cell Analysis of Organoid Signalling Networks (Nature Protocols)

• Metal Antibody Conjugation

SIGNAL-seq (Split-pool Indexing siGNalling AnaLysis by sequencing)

• SIGNAL-seq Oligos

• SIGNAL-seq Oligo Plate Generation

• SIGNAL-seq ADT Staining

• SIGNAL-seq Split Pool Barcoding

• SIGNAL-seq Library Preperation

• SIGNAL-seq Key Reagents

Code

• TAPE Lab GitHub

• CyGNAL (CyTOF siGNalling AnaLysis pipeline )

• SplitRtools (SPLiT-seq data analysis pipeline)

• Automated Barcode Pipetting (for OT-2 platform)

• REPO (Robotic Extraction Protocol for Organoids)

• Trellis (in collaboration with Krishnaswamy Lab) (Kaggle tutorial)